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Based on the principle that acid fuchsin and picric acid have different affinity to collagen fiber and muscle fiber respectively, the collagen fiber was stained double.Collagen fibers are stained red to pink under the action of acid fuchsin, and muscle fibers combine with picric acid to stain yellow.
Mainly used for the identification and staining of collagen fibers and muscle fibers in histocytology
Reagent |
Main Composition |
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Solution A: Hematoxylin solution |
Hematoxylin |
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Solution B1: Van Gieson solution |
Acid fuchsin |
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Solution B2: Van Gieson solution |
Saturated picric acid |