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MUC-5AC Antibody Reagent(IHC)-IFU
2024-07-30[Product Name]
MUC-5AC Antibody Reagent (Immunohistochemical)
[Specification]
1mL/ Vial, 3mL/ Vial, 6mL/ Vial.
[Intended Use]
This reagent is used for immunohistochemical staining based on routine staining to provideauxiliary information for physicians in diagnosis. This reagent was used for the qualitative determination of MUC-5AC antigen in 10% neutral buffered formalin fixed paraffin-embedded tissue section cells. The test results are for clinical reference only. Any interpretation of positive or negative results should be made by thepathologist in combination with path morphology, clinical manifestations and other test methods, and should not be used as a separate diagnostic indicator.
[Principle of Detection]
Based on the principle of specific binding by the complementary function and affinity ofimmunological antigens and antibodies in molecular biology, and through the Redox Reaction, theenzyme labeled antibody catalyzes the color development of chromogenic agents to display theantigens in tissue cells, and to locate the antigens. First, the mouse anti-human MUC-5ACimmunohistochemical monoclonal antibody coupled with MUC-5AC antigen on the tissue. Secondly, the enzyme-labeled sheep anti-mouse/rabbit IgG polymer recognizes MUC-5ACantibodies that have been attached. Thirdly, adding color substrates, horseradish peroxidase on thepolymer can catalyze the decomposition of H2O2 in the chromogenic solution of DAB, thusbenzidine is oxidized into Biphenyl imide, and yellow or brownish yellow staining appears on theantigen sites in the tissue sections. Finally, the samples were re-stained and sealed. The presenceand condition of MUC-5AC on the tissue sections were inferred by observing the colorationsituation through the microscope. As a quality control, paraffin-embedded human stomach tissuesfixed with 10% neutral buffered formalin were used as positive controls. The cell stain is locatedin the cytoplasm.
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