-
Home
-
Product
-
IMMUNOHISTOCHEMISTRY
-
INSTRUMENT
-
MOLECULAR
-
HISTOLOGY
-
CYTOLOGY
-
DIGITAL PATHOLOGY
-
-
Solution
-
Support
-
About
-
Contact
Human Chorionic Gonadotropin Antibody Reagent (IHC)-IFU
2022-05-20[Product Name]
Human Chorionic Gonadotropin Antibody Reagent (Immunohistochemical)
[Specification]
1mL/ Vial, 3mL/ Vial, 6mL/ Vial. [Intended Use] This reagent is used for immunohistochemical staining based on routine staining to provideauxiliary information for physicians in diagnosis. This reagent was used for the qualitative determination of Human Chorionic Gonadotropin antigenin 10% neutral buffered formalin fixed paraffin-embedded tissue section cells. The test results arefor clinical reference only. Any interpretation of positive or negative results should be made by thepathologist in combination with path morphology, clinical manifestations and other test methods, and should not be used as a separate diagnostic indicator.
[Principle of Detection]
Based on the principle of specific binding by the complementary function and affinity ofimmunological antigens and antibodies in molecular biology, and through the Redox Reaction, theenzyme labeled antibody catalyzes the color development of chromogenic agents to display theantigens in tissue cells, and to locate the antigens. First, the mouse anti-human Human ChorionicGonadotropin immunohistochemical monoclonal antibody coupled with Human ChorionicGonadotropin antigen on the tissue. Secondly, the enzyme-labeled sheep anti-mouse/rabbit IgGpolymer recognizes Human Chorionic Gonadotropin antibodies that have been attached. Thirdly, adding color substrates, horseradish peroxidase on the polymer can catalyze the decomposition ofH2O2 in the chromogenic solution of DAB, thus benzidine is oxidized into Biphenyl imide, andyellow or brownish yellow staining appears on the antigen sites in the tissue sections. Finally, thesamples were re-stained and sealed. The presence and condition of Human ChorionicGonadotropin on the tissue sections were inferred by observing the coloration situation throughthe microscope. As a quality control, paraffin-embedded placenta tissues fixed with 10% neutral buffered formalin were used as positive controls. The cell stain is located in the cytoplasm.
Download
