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Home Service & Support Fluorescent In Situ Hybridization Techniques in Pathology

Fluorescent In Situ Hybridization Techniques in Pathology

2021-02-26

By snt_manager

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Advantages of FISH

  • No need of cell culture.
  • FISH can be done on paraffin cell blockmaterial.
  • Archival tissue can be used for FISH.
  • Morphology of the cell can be seenalong with cytogenetic abnormalities.
  • High resolution.
  • The slide can be stored for long time.
  • Fluorescent tags are safe and simple.

 

Limitations of FISH: FISH has following limitations:

  • The FISH technique can be used only in thecase of known chromosomal abnormalities aswe use only the specific probes.
  • It is not suitable for a screening test as onlyknown chromosomal probes are used,whereas in cell culture or conventional technique, we may get a wide range of chromosomal abnormalities.
  • FISH does not give any allele-specificinformation.

 

Applications of FISH:

  • Gain and loss of chromosome: FISH is helpful to detect total gain or loss of chromosomesuch as trisomy 12 in chronic lymphocytic leukaemia which can be detected by FISH in cytology or histology sample.
  • Chromosomal rearrangements: FISH is helpful to identify typical chromosomaltranslocation such as t (11; 22) (q24; q12) inEwing’s/primitive neuroectodermal tumor [4].
  • Gene amplification: Gene amplification suchas HER-2 in breast carcinoma can be detectedby FISH.
  • Gene deletion: Gene deletion such as 9p21deletion in urothelial cell carcinoma can be detected by FISH.
  • Disease monitoring: To assess the progressionor regression of disease and also to identify the minimal residual disease.

More details about FISH probes  https://www.celnovte.com/product-center/?cat=10

Contact us info@celnovte-bio-tech.com

Reference

Pranab Dey, Basic and Advanced Laboratory Techniques in Histopathology and Cytology (2018): 213-214