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Home Service & Support Cytokeratin5&6 Antibody Reagent (IHC)-IFU

Cytokeratin5&6 Antibody Reagent (IHC)-IFU

2024-08-08

By snt_manager

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[Product Name] Cytokeratin5&6 Antibody Reagent (Immunohistochemical)

[Specification] 1mL/ Vial, 3mL/ Vial, 6mL/ Vial.

[Intended Use] This reagent is used for immunohistochemical staining based on routine staining to provideauxiliary information for physicians in diagnosis. This reagent was used for the qualitative determination of cytokeratin5&6 antigen in 10% neutral buffered formalin fixed paraffin-embedded tissue section cells. The test results are for clinical reference only. Any interpretation of positive or negative results should be made by thepathologist in combination with path morphology, clinical manifestations and other test methods, and should not be used as a separate diagnostic indicator.

[Principle of Detection] Based on the principle of specific binding by the complementary function and affinity ofimmunological antigens and antibodies in molecular biology, and through the Redox Reaction, theenzyme labeled antibody catalyzes the color development of chromogenic agents to display theantigens in tissue cells, and to locate the antigens. First, the mouse anti-human cytokeratin5&6immunohistochemical monoclonal antibody coupled with cytokeratin5&6 antigen on the tissue. Secondly, the enzyme-labeled sheep anti-mouse/rabbit IgG polymer recognizes cytokeratin5&6antibodies that have been attached. Thirdly, adding color substrates, horseradish peroxidase on thepolymer can catalyze the decomposition of H2O2 in the chromogenic solution of DAB, thusbenzidine is oxidized into Biphenyl imide, and yellow or brownish yellow staining appears on theantigen sites in the tissue sections. Finally, the samples were re-stained and sealed. The presenceand condition of cytokeratin5&6 on the tissue sections were inferred by observing the colorationsituation through the microscope. As a quality control, paraffin-embedded human esophageal squamous cell carcinoma tissues fixed with 10% neutral buffered formalin were used as positivecontrols. The cell stain is located in the cytoplasm.

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