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CD68 Antibody Reagent(IHC)-IFU
2024-07-30[Product Name]
CD68 Antibody Reagent (Immunohistochemical)
[Specification]
1mL/ Vial, 3mL/ Vial, 6mL/ Vial.
[Intended Use]
Thisreagent is used for immunohistochemicalstaining based on routine staining to provide auxiliary information for physicians in diagnosis. This reagent was used for the qualitative determination of CD68 antigen in 10% neutral buffered formalin fixed paraffin-embedded tissue section cells. The test results are for clinical reference only. Any interpretation of positive or negative results should be made by the pathologist in combination with path morphology, clinical manifestations and other test methods, and should not be used as a separate diagnostic indicator.
[Principle of Detection]
Based on the principle of specific binding by the complementary function and affinity of immunological antigens and antibodies in molecular biology, and through the Redox Reaction, the enzyme labeled antibody catalyzes the color development of chromogenic agents to display the antigens in tissue cells, and to locate the antigens. First, the mouse anti-human CD68 immunohistochemical monoclonal antibody coupled with CD68 antigen on the tissue. Secondly, the enzyme-labeled sheep anti-mouse/rabbit IgG polymer recognizes CD68 antibodies that have been attached. Thirdly, adding color substrates, horseradish peroxidase on the polymer can catalyze the decomposition of H2O2 in the chromogenic solution of DAB, thus benzidine is oxidized into Biphenyl imide, and yellow or brownish yellow staining appears on the antigen sites in the tissue sections. Finally, the samples were re-stained and sealed. The presence and condition of CD68 on the tissue sections were inferred by observing the coloration situation through the microscope. As a quality control, paraffin-embedded tonsil tissues fixed with 10% neutral buffered formalin were used as positive controls. The cell stain is located in the cell membrane and cytoplasm.
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