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CD30 Antibody Reagent(IHC)-IFU
2024-08-03[Product Name] CD30 Antibody Reagent (Immunohistochemical)
[Specification] 1mL/ Vial, 3mL/ Vial, 6mL/ Vial.
[Intended Use] This reagent is used for immunohistochemical staining based on routine staining to provideauxiliary information for physicians in diagnosis. This reagent was used for the qualitative determination of CD30 antigen in 10% neutral bufferedformalin fixed paraffin-embedded tissue section cells. The test results are for clinical referenceonly. Any interpretation of positive or negative results should be made by the pathologist incombination with path morphology, clinical manifestations and other test methods, and should not be used as a separate diagnostic indicator.
[Principle of Detection] Based on the principle of specific binding by the complementary function and affinity ofimmunological antigens and antibodies in molecular biology, and through the Redox Reaction, theenzyme labeled antibody catalyzes the color development of chromogenic agents to display theantigens in tissue cells, and to locate the antigens. First, the mouse anti-human CD30immunohistochemical monoclonal antibody coupled with CD30 antigen on the tissue. Secondly, the enzyme-labeled sheep anti-mouse/rabbit IgG polymer recognizes CD30 antibodies that havebeen attached. Thirdly, adding color substrates, horseradish peroxidase on the polymer cancatalyze the decomposition of H2O2 in the chromogenic solution of DAB, thus benzidine isoxidized into Biphenyl imide, and yellow or brownish yellow staining appears on the antigen sitesin the tissue sections. Finally, the samples were re-stained and sealed. The presence and conditionof CD30 on the tissue sections were inferred by observing the coloration situation through the Henan Celnovte Biotechnology , Ltd. microscope. As a quality control, paraffin-embedded anaplastic large cell lymphoma tissues fixedwith 10% neutral buffered formalin were used as positive controls. The cell stain is located in thecell membrane.
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