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HE staining is one of the commonly used staining methods in paraffin or frozen section technology. Hematoxylin is divided into retrograde and progressive according to the staining method. Now the common hematoxylin in the market is retrograde hematoxylin, that is, hematoxylin is overstained, and then the excess hematoxylin is stained by the differentiation solution.
Hematoxylin-Eosin staining (HE staining) section technology is one of the commonly used staining methods in section technology. Progressive hematoxylin does not require differentiation fluid to be dedifferentiated its staining time is short only 1 minute. The staining solution is stable without metal oxide film and precipitation. It can be used for tissue paraffin section, frozen section and tissue cell staining and reused.
a)High concentration and short staining time for 1 minute
b)No metal oxide film
c)Staining bright blue
d)No need to be differentiated, return directly to blue
a)Fast effect
b)Blue buffer is light blue, easy to identify
Eosin staining solution
Eosin is an acid melt, which mainly makes the components in the cytoplasm and extracellular matrix stain red. Celnovte Bio-tech adopts self-optimized formulation and divides into two types according to customer needs.
1. Water Soluble Eosin
Features: Staining time for 1-3 min, light color, strong contrast with hematoxylin
2. Alcohol Soluble Eosin
Features: Strong staining ability, short staining time, bright color
Shelf Life: 12 months
Storage: 15-30℃
Directions: see inside the box for details
Hematoxylin | Blue Buffer | Water Soluble Eosin | Alcohol Soluble Eosin |
0.5min-1min | 1min | 2-3min | 10-30s |