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Home Product IMMUNOHISTOCHEMISTRY MICROSTACKER™ DETECTION SYSTEMS MicroStacker™ Plus HRP-Polymer Detection Kit

MicroStacker™ Plus HRP-Polymer Detection Kit

Introducing the MicroStacker™ Polymer Detection System, a groundbreaking solution for precise immunohistochemistry (IHC) procedures on formalin-fixed, paraffin-embedded (FFPE) tissues. This biotin-free, polymeric horseradish peroxidase (HRP)-secondary antibody conjugate system is specifically designed for the detection of mouse and rabbit primary antibodies.

Product Features

Summary

Powered by innovative MicroStacker™ technology, this system enables controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. The result? A compact polymeric structure that effortlessly permeates all cellular compartments, ensuring comprehensive staining coverage.

 

Notably, our system utilizes F(ab’) fragments of IgG secondary antibodies, mitigating background noise caused by non-specific binding of whole IgG to endogenous Fc receptors. By upgrading the degree of polymerization of enzymes and antibodies, we achieve heightened sensitivity for optimal detection.

 

Compatible with various automatic immunohistochemistry stainers, our MicroStacker™ Plus HRP-polymer detection kit delivers consistent and reliable results across mouse and rabbit primary antibodies. Elevate your IHC workflow with precision and efficiency using the MicroStacker™ Polymer Detection System.

 

MicroStacker technology

 

  • Unique Polymer Design

The innovative MicroStackerTM technology allows for the orientation-controlled attachment of Fab’ fragments of IgG on the poly-HRP core based on micro-polymer scaffolds.

 

  • Enhanced Sensitivity

The proprietary Fab’ labeling methods avoids the occasional blocking of the antibody binding site during the bioconjugation process, thus increase the sensitivity of the polymer. The compact polymeric structure easily penetrates to all cellular compartments, which provides consistent results in all types of nuclear, cytoplasmic and membranous antigens. The overall effect is superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones.

 

  • Enhanced Specificity

Biotin-free detection eliminates background staining due to endogenous biotin. Plus, the system utilizes Fab’ fragments instead of the whole IgG, which avoids the background caused by non-specific binding of the whole IgG to the endogenous Fc receptor.

 

 

Specification

Code

Product Name

Components

Specification

SD5600

MicroStackerTM Plus HRP-Polymer Detection Kit

Peroxidase Blocking Reagents

30ml*1

Linker, Rabbit-anti-Mouse

30ml*1

Anti-Rabbit HRP-Polymer

30ml*1 (300T)

20*DAB Chromogen

2.4ml*1

DAB Substrate Buffer

30ml*2

Hematoxylin

30ml*1

More Info

Microstacker technology

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