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Alcian Blue – PAS Stain Kit

Alcian blue (AB) staining can be combined with PAS staining method to identify neutral and acid mucins in the same tissue section. Normal mucosal epithelial cells can secrete mucus, which is divided into neutral mucus and acid mucus. They cannot be divided in the HE staining but common mucous staining can. Under pathological conditions, organs for example stomach, intestine, connective tissue, myocardium, kidney, as son on, may also have mucous degeneration, mucous edema, and mucin increase, which also requires staining and certification of mucus substances. Mucus staining is often required for observation and identification in the diagnosis and research of some tumors.

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Product Features

Staining Principle

Alcian blue (AB) is the most specific dye showing acidic mucus substances. This cationic stain forms the salt linkage combined with acidic groups, that is, Alcian blue forms the insoluble complexes with anionic groups such as carboxyl groups and sulfate groups contained in tissues.
The salt linkage of molecule combine with the negative acid groups of acid polysaccharide material to form an insoluble complex and appear blue, and then complex staining with PAS can show three different mucus substance components.

Product Advantages

a)Great Stability

b)Bright Stain Color

Specification

Components

Name		Main Composition
Alcian Blue Stain		Alcian Blue
Periodic Acid Solution		Periodic Acid
Schiff solution		Magenta
Hematoxylin solution		Hematoxylin

More Info

Staining Results : acidic mucus material is blue, neutral mucus material is red, mixed mucus material is blue-violet, and cell nuclei are light blue